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Bacterial growth of S . aureus and E . coli assessed by OASTS. Bacteria were incubated with GA to establish MIC from two-fold serial dilutions. ( A ) The <t>oCelloScope™</t> instrument recorded pictures of the bacterial growth every 10–15 min during the experiment. Pictures from the cultures show the bacterial density at the start (0 h), in the middle (3 h) and at the end (5 h) of the experiments. ( B ) Bacterial growth was measured by the oCelloScope segmentation and extraction of surface area (SESA) algorithm. The applied GA concentrations are indicated in the legend. All data shown represent the mean value and SEM of three independent experiments, each in replicates of two.
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Bacterial growth of S . aureus and E . coli assessed by OASTS. Bacteria were incubated with GA to establish MIC from two-fold serial dilutions. ( A ) The oCelloScope™ instrument recorded pictures of the bacterial growth every 10–15 min during the experiment. Pictures from the cultures show the bacterial density at the start (0 h), in the middle (3 h) and at the end (5 h) of the experiments. ( B ) Bacterial growth was measured by the oCelloScope segmentation and extraction of surface area (SESA) algorithm. The applied GA concentrations are indicated in the legend. All data shown represent the mean value and SEM of three independent experiments, each in replicates of two.

Journal: Scientific Reports

Article Title: The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria

doi: 10.1038/s41598-017-15969-3

Figure Lengend Snippet: Bacterial growth of S . aureus and E . coli assessed by OASTS. Bacteria were incubated with GA to establish MIC from two-fold serial dilutions. ( A ) The oCelloScope™ instrument recorded pictures of the bacterial growth every 10–15 min during the experiment. Pictures from the cultures show the bacterial density at the start (0 h), in the middle (3 h) and at the end (5 h) of the experiments. ( B ) Bacterial growth was measured by the oCelloScope segmentation and extraction of surface area (SESA) algorithm. The applied GA concentrations are indicated in the legend. All data shown represent the mean value and SEM of three independent experiments, each in replicates of two.

Article Snippet: Growth was analysed in Nunc Edge™ 96-well plates (Thermo Fisher) by OASTS using the oCelloScope™ instrument (Philips BioCell A/S, Allerød, Denmark).

Techniques: Incubation